Green Synthesis of Nanoparticles and their Antibacterial Activity Against Pathogenic Bacteria

Abstract

This work examined the phytochemical characterization of the primary bioactive ingredients of Punica granatum peels in its aqueous extract, green production of silver nanoparticles, and their antibacterial efficacy. Flavonoids, phenol, tannins, carbohydrates, glycosides, etc. were abundant in peel extract. UV-Visible spectroscopy verified silver nanoparticle production and characterization. UV-Visible spectroscopy of silver nanoparticle-containing reaction media shows maximal absorbance peaks at 430nm (1%), 373nm (3%), and 379nm (5%). The disc diffusion technique was used to assess the green production of silver nanoparticles against bacterial cultures. The silver nanoparticles killed E. coli (MTCC-40), Staphylococcus aureus (MTCC-7443), and Proteus vulgaris (MTCC-*1771). Punica granatum peel extract rapidly lowers Ag+ to Ago and aids silver nanoparticle formation with antimicrobial properties.