Journal of Drug Discovery and Therapeutics https://www.jddt.in/index.php/jddt <p><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><span style="text-align: justify;"><strong>(Scientific Journal Impact Factor Value for 2021)</strong></span></span></span></p> <p><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><span style="text-align: justify;"><strong>SJIF 2021 = 6.104 </strong></span></span></span></p> <p><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><span style="text-align: justify;"><strong>Journal of Drug Discovery and Therapeutics (JDDT)</strong> is an international, peer-reviewed, open access, online journal dedicated to the rapid publication of full-length original research papers, short communications, invited reviews, Case studies and editorial commentary and news, Opinions &amp; Perspectives and Book Reviews written at the invitation of the Editor in all areas of the Biomedical and Pharmaceutical Sciences.</span></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Medical || Dentistry || Biomedical Sciences || Ayurveda || Homeopathy || </strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Anatomy, Physiology, Biochemistry, Molecular Biology, Cell biology, Genetics, Hematology, Pathology, Immunology, Microbiology, Virology, Parasitology, Surgery, Dental Sciences, Sports Physiology, Histopathology, Toxicology and all major disciplines of Biomedical Sciences.<br /><strong>Pharmaceutical Sciences || Allied Sciences </strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Toxicology, Pharmaceutical and Biomedical Analysis, Clinical Research, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology and all major disciplines of Pharmaceutical Sciences.</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Articles are published as they are accepted and are freely available on the journal’s website to facilitate rapid and broad dissemination of research findings to a global audience.</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Top Reasons for publication with us</strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Quick Quality Review:</strong> The journal has strong international team of editors and reviewers, Rapid Decision and Publication</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Very Low Publication Fees:</strong> Comparable journals charge a huge sum for each accepted manuscript. JDDT only charge the fees necessary to recoup cost associated with running the journal</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Other features:</strong> DIDS Assigned and Implemented the Open Review System (ORS).</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Important Notice:</strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Author can now directly send their manuscript as an email attachment to</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Innovative Library</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>editor@jddt.in</strong>, <strong>editorjddt.in@gmail.com</strong></span></span></p> <p> </p> en-US editor@jddt.in (JDDT-PUBLISHER) INNOVATIVELIBRARY6@GMAIL.COM (INN. LIB) Fri, 07 Jun 2024 12:56:32 +0000 OJS 3.2.1.1 http://blogs.law.harvard.edu/tech/rss 60 Neuroprotective Effect Of Cymbopogon nardus And Polygonum glabrum On Alcohol-Induced Alzheimer In Rats https://www.jddt.in/index.php/jddt/article/view/555 <p style="font-weight: 400;">Alzheimer's disease (AD) is a degenerative neurological condition that gradually worsens over time. It is marked by a decline in cognitive function, including memory loss, and changes in behaviour. AD aetiology is influenced by several variables, including genetics, environment, and lifestyle. Alcohol use is a risk factor that may be changed and has the potential to impact the structure and function of the brain. Prolonged alcohol use may cause neuroinflammation, oxidative stress, and neuronal death, resulting in cognitive impairment and dementia. Nevertheless, the impact of alcohol on the developing brain remains little understood. This research examined the effects of alcohol on the brains of adult rats and explored the possible therapeutic benefits of extracts from Cymbopogon nardus L. and Polygonum glabrum L. for treating Alzheimer's disease. We used a rat model to simulate the drinking behaviour of human adolescents, namely binge ethanol consumption. The rats were subjected to subcutaneous administration of ethanol at a dosage of 2 g/kg every day for a duration of 21 days. Subsequently, rats who had received treatment were exposed to behavioural and biochemical assessments. Our investigation revealed that excessive ethanol consumption has a detrimental effect on the ability of adult rats to acquire and remember spatial information, as shown by the Morris water maze tests. Ethanol furthermore decreased the number of dendritic spines and synaptic proteins in both the hippocampus and cortex, which suggests the occurrence of synaptic injury. In addition, ethanol elevated the concentrations of pro-inflammatory cytokines, oxidative stress indicators, and apoptotic proteins in the brain regions. The administration of extracts effectively corrected the cognitive impairments, loss of synaptic connections, inflammation of brain tissue, oxidative damage, and death of neurones caused by ethanol exposure. The findings of our study indicate that exposing teenage rats to binge alcohol leads to persistent neurodegeneration and cognitive impairment in adulthood. However, these negative effects may be mitigated by the administration of BMCT, AMCT, WCO, and HAEPG. This work offers novel perspectives on the processes behind alcohol-induced brain damage and the potential advantages of using extracts as a therapy to prevent or manage alcohol-related dementia.Utilising any metric to evaluate dementia clinically, whether in individuals with cognitive impairments or in the general population, is inherently constrained. Having a thorough understanding of these limitations allows us to make informed decisions in selecting appropriate scientific methods to customise our neuropsychological assessment or consider alternate measures.</p> <p style="font-weight: 400;">In the end, there could be a middle ground due to these constraints; yet, scientific knowledge has provided us with a more comprehensive understanding of the progression of dementia than ever before. By using advanced technologies like MRI, fMRI, PET, and SPET scans, together with cognitive testing conducted at certain intervals, including follow-ups, clinicians now have a greater ability to provide a more accurate diagnosis and prognosis compared to previous methods. It is expected that this would help educate service providers in expanding access to individuals with learning difficulties who also have dementia.</p> <p><strong>Keywords: </strong><span style="font-weight: 400;">Alzheimer, oxidative stress, ethanol</span></p> Pankaj Singh, Jitendra Malviya Copyright (c) 2024 https://www.jddt.in/index.php/jddt/article/view/555 Fri, 31 May 2024 00:00:00 +0000 Medicinal Plants with Hepatoprotective Activity: A Systematic Review https://www.jddt.in/index.php/jddt/article/view/556 <p style="font-weight: 400;">Liver diseases such as hepatitis, fatty liver, cirrhosis, and liver cancer are significant global health concerns, resulting from viral infections and exposure to substances that are harmful to the liver. Natural products are crucial in the identification of several novel medications and active components. Medicinal herbs and herbal mixtures are used to treat a variety of liver problems. However, in extreme circumstances, the efficacy of a single medicinal plant is not very satisfying. These plants possess potent medicinal compounds such as alkaloids, glycosides, saponins, flavonoids, antioxidants, and terpenes that have hepatoprotective properties against harmful substances.</p> <p style="font-weight: 400;">Silibum marianum, also known as milk thistle, contains Silymarin which has proven efficacy against specific liver diseases. <em>Glycyrrhiza</em> <em>glabra</em>, or Glycyrrhizin, Picrorhiza kurroa with picrosides I and II, <em>Phyllanthus</em> maderaspatensis with β-sitosterol, Andrographis paniculata with Andrographolide, <em>Trichopus zeylanicus, Eclipta alba, and Phyllanthus amarus</em> are also effective against certain liver diseases.</p> <p><strong>Keywords: </strong>Hepatoprotective, hepatitis, medicinal plants, phytochemicals</p> Krishna Anand, Jitendra Malviya Copyright (c) 2024 https://www.jddt.in/index.php/jddt/article/view/556 Thu, 30 May 2024 00:00:00 +0000 Simultaneous Enantioseparation and Simulation Studies of Some Drugs using Supercritical Fluid Chromatography https://www.jddt.in/index.php/jddt/article/view/557 <p style="font-weight: 400;">The enantioseparation of three b-blockers, namely atenolol, metoprolol, and propranolol, was investigated using supercritical fluid chromatography (SFC) on a chiral stationary phase immobilised with amylose tris(3-chloro-5-methylphenylcarbamate). An assessment was conducted to determine the impact of organic modifiers (methanol, isopropanol, and their combination), column temperature, and back pressure on the chiral separation of b-blockers. The best chromatographic separation in terms of resolution, retention, and analysis duration was obtained by utilising a combination of CO2 and 0.1% isopropyl amine in isopropanol: methanol (50:50, V/V), in a ratio of 75:25 (V/V).</p> <p>When the settings were optimised, the resolution factors (Rs) and separation factors (a) were both higher than 3.0 and 1.5, respectively. Moreover, when the temperature (25e45 ◦C) and pressure (100e150 bars) increased, there was a commensurate drop in the retention factors (k), a, and Rs. Conversely, there was an opposite pattern found with atenolol as the temperature increased. The thermodynamic data obtained from van't Hoff plots indicated that the separation of enantiomers was primarily driven by changes in enthalpy for metoprolol and propranolol, while it was driven by changes in entropy for atenolol. Molecular docking experiments were conducted to provide insight into the process of chiral recognition and the elution behaviour of the enantiomers. The binding energies derived from simulation studies shown strong concordance with both the empirically determined elution sequence and the corresponding free energy values. The approach was verified within the concentration range of 0.5e10 mg/mL for all enantiomers. The range for the limit of detection was 0.126 to 0.137 mg/mL, while the range for the limit of quantitation was 0.376 to 0.414 mg/mL. The approach was effectively used to analyse these substances in pharmaceutical formulations.</p> Nidhi Katiyar, Arun Sharma Copyright (c) 2024 https://www.jddt.in/index.php/jddt/article/view/557 Thu, 30 May 2024 00:00:00 +0000 A Review on Pharmacological Properties of Acorus Calamus https://www.jddt.in/index.php/jddt/article/view/558 <p>Ayurveda has the potential to be a valuable source of new anti-inflammatory substances for the treatment of chronic illnesses. This study aims to offer a comprehensive overview of a significant ayurvedic plant and its therapeutic use in diverse ailments. It is used in treating conditions such as bronchitis, nerve disorders, colic, chest discomfort, diarrhoea, flatulence, indigestion, rheumatism, cough, fever, depression, tumours, skin diseases, works as a cryoprotective agent, inflammation, and other neurological disorders. It has antimicrobial, anticancer, and maybe antidiabetic properties. Several active phytoconstituents have been identified and described from the leaves and rhizomes, which contain significant essential oils. The rhizome component of the plant has significant medicinal properties. The plant has two main bioactive chemicals, α-asarone and β-asarone, which are the primary constituents. The usage of α- and β-asarone is limited due to the genotoxicity and mutagenicity seen in several investigations at higher concentrations. The understanding of the active chemical components of Ayurvedic plants and the specific inflammatory pathways they suppress is still unclear.</p> <p><strong><em>Keywords</em>: </strong><span style="font-weight: 400;">Acorus, Diseases, Protection </span></p> Vibhor Sharma, Jitendra Malviya Copyright (c) 2024 https://www.jddt.in/index.php/jddt/article/view/558 Fri, 31 May 2024 00:00:00 +0000 Development of RP-HPLC Method for Simultaneous Estimation of Lornoxicam and Paracetamol in Combined Dosage Forms https://www.jddt.in/index.php/jddt/article/view/560 <p>A simple, rapid reverse phase high-performance liquid chromatographic method was developed and validated for the simultaneous estimation of paracetamol and lornoxicam in bulk and pharmaceutical dosage forms. Determination of the different analytical parameters such as linearity, precision, accuracy, and specificity, limit of detection (LOD) and limit of quantification (LOQ) was done. The calibration curve was found to be linear for each analyte in the desired concentration range. The proposed method is highly sensitive, precise and accurate for paracetamol and lornoxicam respectively and hence the present method can be applied successfully for the quantification of active pharmaceutical ingredient (API) content in the combined formulations of paracetamol and lornoxicam.</p> Vivek Singh Thakur, Amit Kumar Copyright (c) 2024 https://www.jddt.in/index.php/jddt/article/view/560 Thu, 30 May 2024 00:00:00 +0000 Design, Development, and Evaluation of Gelucire-Based Gastric Floating Beads of Pregabalin https://www.jddt.in/index.php/jddt/article/view/561 <p style="font-weight: 400;"><strong>Abstract: </strong>The objective of this research is to design, develop, and evaluate Gelucire-based gastric floating beads of Pregabalin. These beads are intended to provide a sustained release profile, enhance bioavailability, and improve patient compliance. The beads were prepared using the ionotropic gelation technique and characterized for their physicochemical properties, buoyancy, drug entrapment efficiency, and in vitro release profile. The results demonstrated that the optimized formulation exhibited a prolonged release of Pregabalin, enhanced floating ability, and improved bioavailability compared to conventional formulations.</p> <p style="font-weight: 400;">An attempt is made to prepare bead of 210nm using various grades of gelucire such as Gelucire 50/13, Gelucire 48/01, Gelucire 43/01. Among which gelucire 43/01 gave spherical bead. The method of preparation of beads was found to be simple and reproducible. Percentage yield was found in a range of 70±1.5 to 89.25±0.28. Percentage drug entrapment of drug was obtained in all formulations in a range of 47.79±1.90 to 87.95±0.75. Due to higher Drug-lipid ratio beads the size of bead slightly increased produce. The average size of bead range was between 2.42±0.13 to 3.24±0.05µm. The in vitro data indicated that pure drug was 99% release with in 3hrs. The drug release from the bead prepared in formulation F8 achieved 60.93±0.56% in 6hrs and 80.06±0.28% in 12hrs.</p> <p><strong>Keywords</strong>: Pregabalin, Gelucire, Gastric floating beads, Sustained release, Bioavailability, Ionotropic gelation.</p> Pradeep Garg, Arindam Chattrejee, Priya Sharma, Sunil Sain Copyright (c) 2024 https://www.jddt.in/index.php/jddt/article/view/561 Thu, 06 Jun 2024 00:00:00 +0000 Evaluation of Antidiabetic Activity of Catharanthus Pusillus on Streptozotocin-Induced Diabetic Albino Wistar Rats https://www.jddt.in/index.php/jddt/article/view/562 <p style="font-weight: 400;">The study aims to evaluate the antidiabetic activity of Catharanthus pusillus in streptozotocin-induced diabetic albino Wistar rats. Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. Despite various synthetic drugs available for its management, natural plant-based remedies are gaining attention for their efficacy and minimal side effects. Catharanthus pusillus has been traditionally used in folk medicine for treating diabetes. This study investigates its efficacy and potential mechanisms in controlling blood glucose levels.</p> <p><strong>Keywords</strong>: Catharanthus pusillus, Antidiabetic activity, Streptozotocin, Diabetes mellitus, Albino Wistar rats.</p> Rajesh Asija, Amandeep Swami, Manoj . Copyright (c) 2024 https://www.jddt.in/index.php/jddt/article/view/562 Thu, 06 Jun 2024 00:00:00 +0000