Evaluation of in-vivo anti-inflammatory activity of Tridax procumbens Linn extract

Abstract

The present study investigates the in-vivo anti-inflammatory potential of various extracts of Tridax procumbens Linn, a medicinal herb traditionally used in Indian folklore for treating inflammation and wounds. The anti-inflammatory activity was evaluated using standard biochemical markers including alanine transaminase (ALT), aspartate transaminase (AST), lipid peroxidase (MDA), and alkaline phosphatase (ALP) in experimental animal models. Different solvent extracts—ethanolic (TP-EtOHE), aqueous (TP-AqE), chloroform (TP-CHCl₃E), and petroleum ether (TP-PEE)—were administered at a dose of 200 mg/kg body weight, while a standardized ethanol extract (TPEE-S) was tested at 30 mg/kg. Diclofenac (50 mg/kg) served as the standard drug. Results indicated that the TPEE-S and TP-EtOHE extracts significantly reduced inflammatory biomarkers, with values comparable to the standard drug, suggesting potent anti-inflammatory properties. The aqueous, chloroform, and petroleum ether extracts also demonstrated moderate activity. These findings support the traditional use of Tridax procumbens in inflammation and warrant further studies to isolate and characterize its active constituents.

Keywords: Tridax procumbens, Anti-inflammatory, In-vivo study, Phytochemicals, Ethanolic extract, Lipid peroxidation, Liver enzymes, Herbal medicine