Preparation, characterization, and antimicrobial evaluation of 5–(4-Morpholin-4-YL)- 3- Nitrobenzene - 1 - Sulfonyl) - 4, 5, 6, 7 -Tetrahydrothieno [3,2-C] Pyridine

Abstract

A novel series of thienopyridine derivatives, particularly 5–(4-morpholin-4-yl)-3-nitrobenzene-1-sulfonyl)-4,5,6,7-tetrahydrothieno[3,2-c] pyridine, was synthesized and evaluated for antimicrobial activity. Structural characterization was carried out using spectral techniques, including IR, NMR, and mass spectrometry. The synthesized compounds were tested against a panel of Gram-positive and Gram-negative bacterial strains (S. aureus, S. pyogenes, E. coli, P. aeruginosa) and fungal species (A. niger) using the broth dilution method to determine minimum inhibitory concentrations (MICs). Among the tested compounds, derivatives with piperazine and piperidine moieties demonstrated better activity profiles compared to morpholine analogs. The findings suggest the potential of these sulfonylated thienopyridines as promising antimicrobial agents with further optimization.

Keywords: Thienopyridine derivatives, Morpholine analog, antimicrobial activity, Broth dilution method, Minimum inhibitory concentration (MIC)