Isolation and Characterization of Phytoconstituent from Nyctanthes arbortristis L.

Abstract

In Indian system of traditional medicine, it is presumed that the knowledge of Ayurveda is given by Gods of a different world. Nyctanthes arbortristis L. is an indigenous plant which is often planted for its beautiful flowers which are used for worship and as an ornament. When quite old, it is a small tree with a crooked trunk. Leaves of Nyctanthes arbortristis L. contain an alkaloidal principle ‘nyctanthine’, also contain mannitol, astringent principles, resinous substances, ascorbic acid, alkaloids (nyctanthine), colouring matters, sugar and traces of an oily substance, tannic acid, methyl salicytate, carotene, an amorphous resin and trace of volatile oil. The shade dried leaf powder was extracted with 95% ethanol and distilled water in continuous hot extraction in soxhlet apparatus. The extract filtered and the solvent evaporated off the mixture with the help of vacuum rotary evaporator. The residue dried in Vacuum oven at 15 mm Hg pressure and at 350C, the residue was found in powder form. Extracted materials further investigated for phytochemical screening by different qualitative test and column chromatography using solvents like acetone and ethanol and water fractions were obtained, further characterization were carried out by different spectral analysis like FT-IR, Mass, 1H-NMR.

Key words- Nyctanthes arbortristis L., alkaloids, nyctanthine, mannitol, Herbal drugs.