Analysis of the Behavior of oil degrading Bacteria Enriched Bioremediation of spilled oil

Abstract

Petroleum hydrocarbon contamination in soil is the major problems resulting from the activities related to petroleum industry, automobile garage/shops, and accidental release in soil. The present thesis work is carried out by isolation, identification, characterization & optimization of spilled oil degrading bacteria from spilled oil contaminated soil sites. Two oil degrading bacteria were isolated from different places of Lucknow, U.P.India. In this work the two oil degrading bacteria i.e. Bacillus cereus and Streptococcus faecalis were isolated, identified and Characterize by colony morphology,staining and biochemical characteristics based on Bergey’s Manual .The used Mobil oil &2T oil is used to check oil degradation activity by the method of the Shake Erlenmeyer Flask Method, Agar well diffusion method & Spread Plate method. The Biosurfactant activity was measured till 7 days and further optimization was carried out for best carbon source, nitrogen source,pH, temperature and again Checked biosurfactant activities. Starch and Sucrose is the best Carbon sources in Bacillus cereus and Streptococcus faecalis respectively. Urea and Peptone is the best Nitrogen sources for Bacillus cereus and Streptococcus faecalis respectively. pH 7 is the best for both Bacillus cereus and Streptococcus faecalis and optimum temperature 370C was best for both cultures.