ISOLATION AND CHARACTERIZATION OF A FLAVONOID FROM ETHANOLIC EXTRACT OF Portulaca quadrifida LINN.

Abstract

The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Portulaca quadrifida Linn. and characterization of the flavonoids by using several chromatographic and analytical methods. Dried aerial part of the plant was extracted by using 95% ethanol in Soxhlet apparatus. The ethanolic extract was used for isolation. TLC was performed to find the no of constituents present in the extract. Column had done to separate the constituent. The fractions giving same spot in TLC were collected, mixed together and crystallized. It was purified and recrystalized for analytical studies (EEPQ-I). FTIR, NMR and MASS had performed for characterizing about the compound EEPQ-I. IR study support the presence of hydroxyl, methoxyl group and saturated ring. The NMR study proved the presence of methine proton in different position of the structure, ring character and no of groups present in the structure. The MASS study gave EI-MS spectrum of molecular ion peak at m/z 272, corresponding to the molecular formula C16H16O4. The available data and on comparison data from literature confirmed the compound as 3´-methoxy - 4´-hydroxy flavan-3-ol.

Key words: Flavonoids, Chromatography, FTIR, NMR, MASS.