A non-traditional therapeutic strategy in management of fulminant hepatic failure: histopathological and immunohistochemistry study

Authors

  • Farid A. Badria Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT
  • Atallah F. Ahmed Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT
  • Wael M. Elsaed Department of Anatomy and Embryology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT
  • Omar M. Gabre Department of Anatomy and Embryology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT
  • Kamal G. Botros Department of Anatomy and Embryology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EGYPT

Keywords:

D-Galactosamine, FHF, Cytokeratine immune stain

Abstract

The present study was undertaken to evaluate the crucial role of hepatocytes transplantation in experimental model of D-galactosamine induced fulminating hepatic failure (FHF) in rat. Hepatocytes were isolated and grown from a group of apparently healthy rats. The isolated hepatocyte was characterized and cultured in vitro and transplanted in FHF rat models either alone or with cyclosporine as immunosuppressive agent. The isolated hepatocytes were cultured for 24 hours prior transplantation in the peritoneal cavity in each of infected (FHF) rat. It was observed that the high survival rate and the improvement of the liver function parameters among transplanted groups versus the non-treated ones.  Histopathological and immunehistochemistry data revealed the presence of hepatocytes attached to the peritoneal covering of the abdominal organs in either a single or several layers.

 

Key words: D-Galactosamine, FHF, Cytokeratine immune stain

Downloads

Published

2014-01-30

Issue

Vol. 2 No. 13 (2014)

Section

Articles