Simultaneous Estimation of Quercetin and Rutin in Aganosma Cymosa by High Performance Liquid Chromatography Method


  • Dipak Thange Research scholar, Department of Pharmacy, Sunrise University, Alwar Rajasthan.
  • Arun Sharma Research supervisor, Department of Pharmacy, Sunrise University, Alwar Rajasthan.
  • Sheetal Kumar Bhong Department of Chemistry, Sinhgad College of Science, Pune-43.


Aganosma cymosa


In order to measure quercetin and rutin in Aganosma cymosa at the same time, a straightforward, specific, accurate, and exact HPLC technique was created. A chromatographic separation was accomplished using a mobile phase consisting of acetonitrile:25mM ammonium acetate pH 3 (40:60 v/v), a C18 column with dimensions 150 x 4.6mm i.d. and a 5µ Hibar Lichrospher. At 259 nm, the absorbance was measured, and the flow rate was 1 ml/min. Rutin had a retention duration of 1.71 minutes and quercetin of 4.30 minutes. The analytical parameters that were used to verify the proposed technique were established according to the recommendations set forth by the International Conference on Harmonisation (ICH). These parameters include accuracy, linearity, precision, robustness, and limit of detection (LOD) and limit of quantification (LOQ). There was a linear relationship between the detector response and the concentrations of quercetin (1–5 µg/ml) and rutin (0.1–0.5 µg/ml). Both components of Aganosma cymosa were accurately and concurrently estimated using the suggested approach. This work developed a quantitative approach to extracting rutin and quercetin from Aganosma cymosa at the same time.

Keywords: Aganosma cymosa, Simultaneous estimation, Flavonoids, Quercetin, Rutin..